Lee, Denise;
Naikar, J Shamita;
Chan, Sophia SY;
Meivita, Maria Prisca;
Li, Lunna;
Tan, Yaw Sing;
Bajalovic, Natasa;
(2022)
Ultralong recovery time in nanosecond electroporation systems enabled by orientational-disordering processes.
Nanoscale
, 14
(21)
pp. 7934-7942.
10.1039/d1nr07362a.
Preview |
Text
Lunna_Ultralong-recovery-time nanosecond electroporation system enabled by orientational-disordering processes (1).pdf - Accepted Version Download (1MB) | Preview |
Abstract
The growing importance of applications based on molecular medicine and genetic engineering is driving the need to develop high-performance electroporation technologies. The electroporation phenomenon involves disruption of the cell for increasing membrane permeability. Although there is a multitude of research focused on exploring new electroporation techniques, the engineering of programming schemes suitable for these electroporation methods remains a challenge. Nanosecond stimulations could be promising candidates for these techniques owing to their ability to generate a wide range of biological responses. Here we control the membrane permeabilization of cancer cells using different numbers of electric-field pulses through orientational disordering effects. We then report our exploration of a few-volt nanosecond alternating-current (AC) stimulation method with an increased number of pulses for developing electroporation systems. A recovery time of ∼720 min was achieved, which is above the average of ∼76 min for existing electroporation methods using medium cell populations, as well as a previously unreported increased conductance with an increase in the number of pulses using weak bias amplitudes. All-atom molecular dynamics (MD) simulations reveal the orientation-disordering-facilitated increase in the degree of permeabilization. These findings highlight the potential of few-volt nanosecond AC-stimulation with an increased number of pulse strategies for the development of next-generation low-power electroporation systems.
Type: | Article |
---|---|
Title: | Ultralong recovery time in nanosecond electroporation systems enabled by orientational-disordering processes |
Location: | England |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.1039/d1nr07362a |
Publisher version: | https://doi.org/10.1039/D1NR07362A |
Language: | English |
Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
UCL classification: | UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS UCL |
URI: | https://discovery.ucl.ac.uk/id/eprint/10152114 |
Archive Staff Only
View Item |