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Ultralong recovery time in nanosecond electroporation systems enabled by orientational-disordering processes

Lee, Denise; Naikar, J Shamita; Chan, Sophia SY; Meivita, Maria Prisca; Li, Lunna; Tan, Yaw Sing; Bajalovic, Natasa; (2022) Ultralong recovery time in nanosecond electroporation systems enabled by orientational-disordering processes. Nanoscale , 14 (21) pp. 7934-7942. 10.1039/d1nr07362a. Green open access

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Abstract

The growing importance of applications based on molecular medicine and genetic engineering is driving the need to develop high-performance electroporation technologies. The electroporation phenomenon involves disruption of the cell for increasing membrane permeability. Although there is a multitude of research focused on exploring new electroporation techniques, the engineering of programming schemes suitable for these electroporation methods remains a challenge. Nanosecond stimulations could be promising candidates for these techniques owing to their ability to generate a wide range of biological responses. Here we control the membrane permeabilization of cancer cells using different numbers of electric-field pulses through orientational disordering effects. We then report our exploration of a few-volt nanosecond alternating-current (AC) stimulation method with an increased number of pulses for developing electroporation systems. A recovery time of ∼720 min was achieved, which is above the average of ∼76 min for existing electroporation methods using medium cell populations, as well as a previously unreported increased conductance with an increase in the number of pulses using weak bias amplitudes. All-atom molecular dynamics (MD) simulations reveal the orientation-disordering-facilitated increase in the degree of permeabilization. These findings highlight the potential of few-volt nanosecond AC-stimulation with an increased number of pulse strategies for the development of next-generation low-power electroporation systems.

Type: Article
Title: Ultralong recovery time in nanosecond electroporation systems enabled by orientational-disordering processes
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1039/d1nr07362a
Publisher version: https://doi.org/10.1039/D1NR07362A
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10152114
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