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A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells

Sampson, O; Lim, N; White, J; Vieira, V; Kløverpris, H; Adland, E; Conlon, C; ... Goulder, P; + view all (2022) A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells. Journal of Immunological Methods , 504 , Article 113263. 10.1016/j.jim.2022.113263. Green open access

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Abstract

Central to sex differences observed in outcome from infection and vaccination is the innate immune response, and specifically production of type I interferons by plasmacytoid dendtiric cells (pDCs), the main producers of IFN-α. Evaluation of IFN-α production by pDCs is therefore critical for studies of innate immune function. However, reliable measurement of pDC IFN-α is hampered by reduced cell yields and cytokine production after cryopreservation or after even short delays in stimulating freshly isolated cells. We here describe a simple yet robust method for measuring IFN-α production in pDCs that preserves cell activation and cytokine production through immediate stimulation of whole blood and subsequent maintenance at 37 °C.

Type: Article
Title: A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells
Location: Netherlands
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.jim.2022.113263
Publisher version: https://doi.org/10.1016/j.jim.2022.113263
Language: English
Additional information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third-party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Keywords: Immune sex differences, Innate immunity, Interferon alpha, Plasmacytoid dendritic cell, Toll-like receptor 7, Dendritic Cells, Female, Flow Cytometry, Humans, Immunity, Innate, Interferon Type I, Interferon-alpha, Male
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10151754
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