Roper, Barnaby WR;
Al-Sayejh, Basmah;
Al-Aufi, Ahmed;
Cuthbert, Gary A;
Lacey, Katie;
Homer-Vanniasinkam, Shervanthi;
Harrison, Michael A;
... Ponnambalam, Sreenivasan; + view all
(2022)
Purification and Analysis of Circulating Lipid Particles.
Methods in Molecular Biology
, 2419
pp. 193-212.
10.1007/978-1-0716-1924-7_12.
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Abstract
Lipid particles found in circulating extracellular fluids such as blood or lymph are essential for cellular homeostasis, metabolism and survival. Such particles provide essential lipids and fats which enable cells to synthesize new membranes and regulate different biochemical pathways. Imbalance in lipid particle metabolism can cause pathological states such as atherosclerosis. Here, elevated low-density lipoprotein (LDL) accumulation leads to fat-filled lesions or plaques in arterial walls. In this chapter, we provide a detailed set of protocols for the rapid and safe purification of lipid particles from human blood using high-speed ultracentrifugation. We provide a detailed set of assays for further analysis of the biochemical and cellular properties of these lipid particles. By combining these assays, we can better understand the complex roles of different lipid particles in normal physiology and disease pathology.
| Type: | Article |
|---|---|
| Title: | Purification and Analysis of Circulating Lipid Particles |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-0716-1924-7_12 |
| Publisher version: | https://doi.org/10.1007/978-1-0716-1924-7_12 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Gradient purification, HDL, Human embryonic kidney 293T (HEK293T) cells, Human umbilical vein endothelial cells (HUVECs), LDL, Lipid particles, SDS-PAGE, VLDL, Atherosclerosis, Humans, Lipid Metabolism, Lipoproteins, LDL, Ultracentrifugation |
| UCL classification: | UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10150856 |
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