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Evaluating VEGF-Induced Vascular Leakage Using the Miles Assay

Brash, James T; Ruhrberg, Christiana; Fantin, Alessandro; (2022) Evaluating VEGF-Induced Vascular Leakage Using the Miles Assay. Methods in Molecular Biology , 2475 pp. 289-295. 10.1007/978-1-0716-2217-9_21. Green open access

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Abstract

Before the endothelial mitogenic activity of the Vascular Endothelial Growth Factor A (VEGF) was described, VEGF had already been identified for its ability to induce vascular leakage. VEGF-induced vascular leakage has been most frequently studied in vivo using the Miles assay, a simple yet invaluable technique that has allowed researchers to unravel the molecular mechanisms underpinning vascular leakage both for VEGF and other permeability inducing agents. In this protocol, a mouse is intravenously injected with Evans Blue dye before VEGF is administered locally via intradermal injection. VEGF promotes vascular leak of serum proteins in the dermis, enabling Evans Blue-labeled albumin extravasation from the circulation and subsequent accumulation in the skin. As the volume of dye extravasation is proportional to the degree of vascular leak, it can be quantified as a proxy measurement of VEGF-induced vascular leakage.

Type: Article
Title: Evaluating VEGF-Induced Vascular Leakage Using the Miles Assay
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-0716-2217-9_21
Publisher version: https://doi.org/10.1007/978-1-0716-2217-9_21
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions.
Keywords: Edema, Evans blue, Miles assay, VEGF, Vascular leakage, Vascular permeability
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10150806
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