Fakhruddin, Kausar Sadia;
Samaranayake, Lakshman Perera;
Hamoudi, Rifat Akram;
Ngo, Hien Chi;
Egusa, Horoshi;
(2022)
Diversity of site-specific microbes of occlusal and proximal lesions in severe- early childhood caries (S-ECC).
Journal of Oral Microbiology
, 14
(1)
, Article 2037832. 10.1080/20002297.2022.2037832.
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Abstract
Background: Severe-early childhood caries (S-ECC) a global problem of significant concern, commonly manifest on the occlusal, and proximal surfaces of affected teeth. Despite the major ecological differences between these two niches the compositional differences, if any, in the microbiota of such lesions is unknown. Methods: Deep-dentine caries samples from asymptomatic primary molars of children with S-ECC (n 19) belonging to caries-code 5/6, (ICDAS classification) were evaluated. Employing two primer pools, we amplified and compared the bacterial 16S rRNA gene sequences of the seven hypervariable regions (V2—V4 and V6—V9) using NGS-based assay. Results: Bray-Curtisevaluation indicated that occlusal lesions (OL) had a more homogeneous community than the proximal lesions (PL) with significant compositional differences at the species level (p = 0.01; R- 0.513). Together, the occlusal and proximal niches harbored 263 species, of which 202 (76.8%) species were common to both, while 49 (18.6%) and 12 (4.6%) disparate species were exclusively isolated from the proximal and occlusal niches, respectively. The most commonl genera at both niches included Streptococcus, Prevotella, and Lactobacillus. S. mutans was predominant in PL (p ≤ 0.05), and Atopobium parvulum (p = 0.01) was predominant in OL. Conclusions: Distinct differences exist between the caries microbiota of occlusal and proximal caries in S-ECC.
| Type: | Article |
|---|---|
| Title: | Diversity of site-specific microbes of occlusal and proximal lesions in severe- early childhood caries (S-ECC) |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1080/20002297.2022.2037832 |
| Publisher version: | https://doi.org/10.1080/20002297.2022.2037832 |
| Language: | English |
| Additional information: | © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Severe-early childhood caries (S-ECC), dentine caries, occlusal-caries lesion, proximal-caries lesion, microbial diversity, PERIODONTAL PATHOGENS, STREPTOCOCCUS-MUTANS, VEILLONELLA-ALCALESCENS, ORAL MICROBIOME, CARIOUS LESIONS, BIOFILMS, CHILDREN, BACTERIA, IDENTIFICATION, ROOT |
| UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10148917 |
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