Kucykowicz, Stephanie;
Amin, Oliver E;
Burton, Alice R;
Swadling, Leo;
Schmidt, Nathalie M;
Zakeri, Nekisa;
Davies, Jessica;
... Pallett, Laura J; + view all
(2022)
Isolation of human intrahepatic leukocytes for phenotypic and functional characterization by flow cytometry.
STAR Protocols
, 3
(2)
, Article 101356. 10.1016/j.xpro.2022.101356.
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Abstract
With the growing appreciation of tissue-resident immunity, studying tissue-specific immune cells contributing to both homeostasis and disease is imperative. Here, we provide a protocol for the isolation of human intrahepatic leukocytes (IHL) maximizing viability, purity, and yield. Our protocol is scalable by tissue weight, allowing for reproducible and efficient IHL liberation suitable for functional characterization, cell isolation, and profiling by flow (or mass) cytometry. Furthermore, we provide a "guide" to determine an expected IHL yield per gram of tissue processed. For complete details on the use and execution of this protocol, please refer to Stegmann et al. (2016), Pallett et al. (2017), Easom et al. (2018), Swadling et al. (2020), Pallett et al. (2020), and Zakeri et al. (2022).
| Type: | Article |
|---|---|
| Title: | Isolation of human intrahepatic leukocytes for phenotypic and functional characterization by flow cytometry |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.xpro.2022.101356 |
| Publisher version: | https://doi.org/10.1016/j.xpro.2022.101356 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third-party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Cell isolation, Flow Cytometry/Mass Cytometry, Health Sciences, Immunology, Single Cell, Cell Separation, Flow Cytometry, Humans, Leukocytes, Lymphocytes |
| UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10148355 |
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