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Continuous single pass diafiltration with alternating permeate flow direction for high efficiency buffer exchange

Tan, Ruijie; Hezel, Fabian; Franzreb, Matthias; (2021) Continuous single pass diafiltration with alternating permeate flow direction for high efficiency buffer exchange. Journal of Membrane Science , 619 , Article 118695. 10.1016/j.memsci.2020.118695. Green open access

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Abstract

Looking at current trends within downstream processing (DSP) of high value bioproducts, it shows that there are ongoing efforts in replacing batch processes by continuous variants. However, a unit procedure which still lacks a simple and compact continuous variant is diafiltration. Here, we present such a single piece of diafiltration equipment achieving continuous buffer exchange of up to 99.90%. The device is composed of a 3D-printed single pass diafiltration (SPDF) module containing two commercial ultrafiltration membranes. While the retentate is flowing through a narrow channel between the two membranes, the channels above and below can supply diafiltration buffer or remove permeate solution. The obtained results illustrate systematically the vulnerability of the device to the effect of concentration polarization at the membrane surface, and that this problem can be strongly reduced using an alternating direction of diafiltration buffer perfusion through the membranes as process inherent backflush. By this, a quasi-stationary operation could be obtained during continuous diafiltration, making the device an interesting option for in-process buffer exchange.

Type: Article
Title: Continuous single pass diafiltration with alternating permeate flow direction for high efficiency buffer exchange
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.memsci.2020.118695
Publisher version: https://doi.org/10.1016/j.memsci.2020.118695
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Diafiltration, continuous processing, single pass tangential flow filtration, buffer exchange, counter-current
UCL classification: UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10148322
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