Al-Nesf, Amna;
Mohamed-Ali, Nada;
Acquaah, Vanessa;
Al-Jaber, Maneera;
Al-Nesf, Maryam;
Yassin, Mohamed A;
Orie, Nelson N;
... Al-Maadheed, Mohammed; + view all
(2022)
Untargeted Metabolomics Identifies a Novel Panel of Markers for Autologous Blood Transfusion.
Metabolites
, 12
(5)
, Article 425. 10.3390/metabo12050425.
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Abstract
Untargeted metabolomics was used to analyze serum and urine samples for biomarkers of autologous blood transfusion (ABT). Red blood cell concentrates from donated blood were stored for 35–36 days prior to reinfusion into the donors. Participants were sampled at different time points post-donation and up to 7 days post-transfusion. Metabolomic profiling was performed using ACQUITY ultra performance liquid chromatography (UPLC), Q-Exactive high resolution/accurate mass spectrometer interfaced with a heated electrospray ionization (HESI-II) source and Orbitrap mass analyzer operated at 35,000 mass resolution. The markers of ABT were determined by principal component analysis and metabolites that had p < 0.05 and met ≥ 2-fold change from baseline were selected. A total of 11 serum and eight urinary metabolites, including two urinary plasticizer metabolites, were altered during the study. By the seventh day post-transfusion, the plasticizers had returned to baseline, while changes in nine other metabolites (seven serum and two urinary) remained. Five of these metabolites (serum inosine, guanosine and sphinganine and urinary isocitrate and erythronate) were upregulated, while serum glycourdeoxycholate, S-allylcysteine, 17-alphahydroxypregnenalone 3 and Glutamine conjugate of C6H10O2 (2)* were downregulated. This is the first study to identify a panel of metabolites, from serum and urine, as markers of ABT. Once independently validated, it could be universally adopted to detect ABT.
| Type: | Article |
|---|---|
| Title: | Untargeted Metabolomics Identifies a Novel Panel of Markers for Autologous Blood Transfusion |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3390/metabo12050425 |
| Publisher version: | https://www.mdpi.com/2218-1989/12/5/425 |
| Language: | English |
| Additional information: | © 2022 MDPI. This is an open access article distributed under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). |
| Keywords: | untargeted metabolomics; autologous transfusion; metabolites; biomarker |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Epidemiology and Health > Applied Health Research UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Epidemiology and Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10148302 |
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