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A permeability assay for mouse intestinal organoids

den Daas, Stijn Aaron; Soffientini, Ugo; Chokshi, Shilpa; Mehta, Gautam; (2022) A permeability assay for mouse intestinal organoids. STAR Protocols , 3 (2) , Article 101365. 10.1016/j.xpro.2022.101365. Green open access

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Abstract

Here, we describe an assay for intestinal permeability in mouse intestinal organoids, although this may also be adapted for other species. Propidium iodide (PI) does not penetrate intact biological membranes and thus cannot enter the lumen of intact organoids. Passage of PI within the lumen can be induced by tight junction disruption or epithelial cell death. This technique measures PI-stained extruded dead cells within the organoid lumen to analyze the effect of insults, toxins, or treatments on intestinal organoid permeability.

Type: Article
Title: A permeability assay for mouse intestinal organoids
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.xpro.2022.101365
Publisher version: https://doi.org/10.1016/j.xpro.2022.101365
Language: English
Additional information: © 2022 The Author(s). Original content in this article is licensed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) Licence (https://creativecommons.org/licenses/by/4.0/).
Keywords: Health Sciences, Model Organisms, Organoids
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inst for Liver and Digestive Hlth
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/10148147
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