Parente-Pereira, AC;
Beatson, RE;
Davies, DM;
Hull, C;
Whilding, LM;
Porter, JC;
Maher, J;
(2022)
Generation and application of TGFβ-educated human Vγ9Vδ2 T cells.
STAR Protocols
, 3
(2)
, Article 101319. 10.1016/j.xpro.2022.101319.
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Abstract
Clinical trials that tested the antitumor activity of γδ T cells have been mostly unsuccessful. To address this, we expanded human Vγ9Vδ2 T cells in TGFβ1, a cytokine which enhances their viability, trafficking properties, and intrinsic antitumor activity. This protocol summarizes the production and in vitro functional characterization of TGFβ1 educated human Vγ9Vδ2 cells and highlights their compatibility with chimeric antigen receptor (CAR) engineering. We also describe in vivo testing of the antitumor activity of these CAR T cells in mice. For complete details on the use and execution of this protocol, please refer to Beatson et al. (2021).
| Type: | Article |
|---|---|
| Title: | Generation and application of TGFβ-educated human Vγ9Vδ2 T cells |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.xpro.2022.101319 |
| Publisher version: | https://doi.org/10.1016/j.xpro.2022.101319 |
| Language: | English |
| Additional information: | ©2022 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Respiratory Medicine UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10147756 |
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