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Methods for the identification and characterization of extracellular vesicles in cardiovascular studies - from exosomes to microvesicles

Davidson, Sean M; Boulanger, Chantal M; Aikawa, Elena; Badimon, Lina; Barile, Lucio; Binder, Christoph J; Brisson, Alain; ... Sluijter, Joost PG; + view all (2022) Methods for the identification and characterization of extracellular vesicles in cardiovascular studies - from exosomes to microvesicles. Cardiovascular Research , Article cvac031. 10.1093/cvr/cvac031. (In press).

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Abstract

Extracellular vesicles (EVs) are nanosized vesicles with a lipid bilayer that are released from cells of the cardiovascular system, and are considered important mediators of intercellular and extracellular communication. Two types of EV of particular interest are exosomes and microvesicles, which have been identified in all tissue and body fluids and carry a variety of molecules including RNAs, proteins, and lipids. EVs have potential for use in the diagnosis and prognosis of cardiovascular diseases and as new therapeutic agents, particularly in the setting of myocardial infarction and heart failure. Despite their promise, technical challenges related to their small size make it challenging to accurately identify and characterize them, and to study EV-mediated processes. Here, we aim to provide the reader with an overview of the techniques and technologies available for the separation and characterization of EVs from different sources. Methods for determining the protein, RNA and lipid content of EVs are discussed. The aim of this document is to provide guidance on critical methodological issues and highlight key points for consideration for the investigation of EVs in cardiovascular studies.

Type: Article
Title: Methods for the identification and characterization of extracellular vesicles in cardiovascular studies - from exosomes to microvesicles
Location: England
DOI: 10.1093/cvr/cvac031
Publisher version: https://doi.org/10.1093/cvr/cvac031
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Cardiovascular diseases, exosomes, extracellular vesicles, micro vesicles, plasma
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Cardiovascular Science > Pre-clinical and Fundamental Science
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Cardiovascular Science
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
URI: https://discovery.ucl.ac.uk/id/eprint/10146645
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