Barral, Duarte C;
Staiano, Leopoldo;
Almeida, Cláudia Guimas;
Cutler, Dan F;
Eden, Emily R;
Futter, Clare E;
Galione, Antony;
... Seabra, Miguel C; + view all
(2022)
Current methods to analyze lysosome morphology, positioning, motility and function.
Traffic
, 23
(5)
pp. 238-269.
10.1111/tra.12839.
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Abstract
Since the discovery of lysosomes more than 70 years ago, much has been learned about the functions of these organelles. Lysosomes were regarded as exclusively degradative organelles, but more recent research has revealed that they play essential roles in several other cellular functions, such as nutrient sensing, intracellular signalling, and metabolism. Methodological advances played a key part in generating our current knowledge about the biology of this multifaceted organelle. In this review, we cover current methods used to analyse lysosome morphology, positioning, motility, and function. We highlight the principles behind these methods, the methodological strategies, and their advantages and limitations. To extract accurate information and avoid misinterpretations, we discuss the best strategies to identify lysosomes and assess their characteristics and functions. With this review, we aim to stimulate an increase in the quantity and quality of research on lysosomes and further ground-breaking discoveries on an organelle that continues to surprise and excite cell biologists.
| Type: | Article |
|---|---|
| Title: | Current methods to analyze lysosome morphology, positioning, motility and function |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1111/tra.12839 |
| Publisher version: | https://doi.org/10.1111/tra.12839 |
| Language: | English |
| Additional information: | © 2022 The Authors. Traffic published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Endolysosomes, Lysosomal storage diseases, Lysosome biogenesis, Lysosome exocytosis, Lysosome-related organelles, Lysosomes, Membrane contact sites, TFEB, mTOR |
| UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10146532 |
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