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Whole-genome sequencing of Chlamydia trachomatis isolates from persistently infected patients

Pitt, Rachel; Doyle, Ronan; Theilgaard Christiansen, Mette; Horner, Paddy; Hathorn, Emma; Alexander, Sarah; Woodford, Neil; ... Breuer, Judith; + view all (2022) Whole-genome sequencing of Chlamydia trachomatis isolates from persistently infected patients. International Journal of STD and AIDS 10.1177/09564624211048662. (In press). Green open access

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Abstract

BACKGROUND: Current understanding of the causes of treatment failure in Chlamydia trachomatis is poor and antimicrobial susceptibility data are lacking. We used genome sequencing to seek evidence of antimicrobial resistance in isolates sourced from patients who were persistently infected. METHODS: Genomic DNA was extracted from C. trachomatis isolates cultured in McCoy cell monolayers. Sequencing libraries were prepared using the SureSelectXT Illumina paired-end protocol. Paired reads were mapped against a reference genome and single nucleotide variants (SNVs) were identified. RESULTS: Seven isolates from persistently infected patients and five isolates from successfully treated patients were sequenced. No previously reported SNVs associated with antimicrobial resistance were found. A unique SNV was identified in the gyrA gene of one treatment failure isolate but was located outside of the quinolone resistance determining region; this SNV has been previously reported in other members of the Chlamydiaceae family. CONCLUSION: No genomic evidence was found to explain the differences in clinical outcome for our two groups of patients. A mutation unrelated to antimicrobial susceptibility was found in an isolate from a persistently infected patient. The cause of these persistent infections with C. trachomatis remains unclear.

Type: Article
Title: Whole-genome sequencing of Chlamydia trachomatis isolates from persistently infected patients
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1177/09564624211048662
Publisher version: https://doi.org/10.1177/09564624211048662
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Chlamydia, antimicrobial, genome sequencing, treatment failure
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
URI: https://discovery.ucl.ac.uk/id/eprint/10145752
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