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A heterogeneous "resting" pool of synaptic vesicles that is dynamically interchanged across boutons in mammalian CNS synapses

Fernandez-Alfonso, Tomas; Ryan, Timothy A; (2008) A heterogeneous "resting" pool of synaptic vesicles that is dynamically interchanged across boutons in mammalian CNS synapses. Brain Cell Biology , 36 (1-4) pp. 87-100. 10.1007/s11068-008-9030-y. Green open access

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Abstract

Using pHluorin-tagged synaptic vesicle proteins we have examined the partitioning of these probes into recycling and nonrecycling pools at hippocampal nerve terminals in cell culture. Our studies show that for three of the major synaptic vesicle components, vGlut-1, VAMP-2, and Synaptotagmin I, approximately 50-60% of the tagged protein appears in a recycling pool that responds readily to sustained action potential stimulation by mobilizing and fusing with the plasma membrane, while the remainder is targeted to a nonrecycling, acidic compartment. The fraction of recycling and nonrecycling (or resting) pools varied significantly across boutons within an individual axon, from 100% resting (silent) to 100% recycling. Single-bouton bleaching studies show that recycling and resting pools are dynamic and exchange between synaptic boutons. The quantitative parameters that can be extracted with the approaches outlined here should help elucidate the potential functional role of the resting vesicle pool.

Type: Article
Title: A heterogeneous "resting" pool of synaptic vesicles that is dynamically interchanged across boutons in mammalian CNS synapses
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/s11068-008-9030-y
Publisher version: https://doi.org/10.1007/s11068-008-9030-y
Language: English
Additional information: Open Access This is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
Keywords: Science & Technology, Life Sciences & Biomedicine, Cell Biology, Neurosciences, Neurosciences & Neurology, CENTRAL-NERVOUS-SYSTEM, HIPPOCAMPAL SYNAPSES, NEUROTRANSMITTER RELEASE, TETANIC STIMULATION, PRESYNAPTIC BOUTONS, DROSOPHILA MUTANT, DISTINCT POOLS, ENDOCYTOSIS, MOBILIZATION, PROTEINS
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
URI: https://discovery.ucl.ac.uk/id/eprint/10145573
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