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The Embryonic Mouse Hindbrain Model to Study Sprouting Angiogenesis In Vivo

D'Amico, Gabriela; Ruhrberg, Christiana; (2022) The Embryonic Mouse Hindbrain Model to Study Sprouting Angiogenesis In Vivo. Methods in Molecular Biology , 2441 pp. 3-18. 10.1007/978-1-0716-2059-5_1. Green open access

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Abstract

Blood vessel growth is a fundamental process for organ development and wound healing but is also associated with ischemic diseases and cancer. The growth of new blood vessels from preexisting vasculature, termed sprouting angiogenesis, is the predominant mode of blood vessel growth in central nervous system vascularization and pathological vessel growth. Accordingly, studying the molecular and cellular mechanisms of angiogenesis holds the promise to find novel therapeutic targets to stimulate new vessel formation in ischemic tissues or inhibit pathological vessel growth in disease. The embryonic mouse hindbrain provides an excellent model to study sprouting angiogenesis in vivo by histochemical or fluorescent wholemount immunolabeling, thus allowing high-resolution image capture of nascent vasculature and subsequent quantification of relevant angiogenic parameters. This chapter describes how to use the mouse embryonic hindbrain as a model to study physiological angiogenesis, including detailed protocols for hindbrain dissection, wholemount staining, and angiogenic parameters analysis.

Type: Article
Title: The Embryonic Mouse Hindbrain Model to Study Sprouting Angiogenesis In Vivo
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-0716-2059-5_1
Publisher version: https://doi.org/10.1007/978-1-0716-2059-5_1
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Hindbrain model, Sprouting angiogenesis, Wholemount immunolabeling
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10145449
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