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Triggered Assembly of a DNA-Based Membrane Channel

Lanphere, Conor; Ciccone, Jonah; Dorey, Adam; Hagleitner-Ertuğrul, Nora; Knyazev, Denis; Haider, Shozeb; Howorka, Stefan; (2022) Triggered Assembly of a DNA-Based Membrane Channel. Journal of the American Chemical Society , 144 (10) pp. 4333-4344. 10.1021/jacs.1c06598. Green open access

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Abstract

Chemistry is in a powerful position to synthetically replicate biomolecular structures. Adding functional complexity is key to increase the biomimetics' value for science and technology yet is difficult to achieve with poorly controlled building materials. Here, we use defined DNA blocks to rationally design a triggerable synthetic nanopore that integrates multiple functions of biological membrane proteins. Soluble triggers bind via molecular recognition to the nanopore components changing their structure and membrane position, which controls the assembly into a defined channel for efficient transmembrane cargo transport. Using ensemble, single-molecule, and simulation analysis, our activatable pore provides insight into the kinetics and structural dynamics of DNA assembly at the membrane interface. The triggered channel advances functional DNA nanotechnology and synthetic biology and will guide the design of controlled nanodevices for sensing, cell biological research, and drug delivery.

Type: Article
Title: Triggered Assembly of a DNA-Based Membrane Channel
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1021/jacs.1c06598
Publisher version: https://doi.org/10.1021/jacs.1c06598
Language: English
Additional information: © 2022 The Authors. Published by American Chemical Society, This is an open access article under the CC BY 4.0 license Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/)
Keywords: Vesicles, Genetics, Membranes, Nanopores, Fluorescence resonance energy transfer
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharma and Bio Chemistry
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
UCL > Provost and Vice Provost Offices > UCL BEAMS
URI: https://discovery.ucl.ac.uk/id/eprint/10145238
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