Michelozzi, Ilaria M;
Sufi, Jahangir;
Adejumo, Thomas A;
Amrolia, Persis J;
Tape, Christopher J;
Giustacchini, Alice;
(2022)
High-dimensional functional phenotyping of preclinical human CAR T cells using mass cytometry.
STAR Protocols
, 3
(1)
, Article 101174. 10.1016/j.xpro.2022.101174.
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Abstract
Here, we present a comprehensive protocol for the generation and functional characterization of chimeric antigen receptor (CAR) T cells and their products by mass cytometry in a reproducible and scalable manner. We describe the production of CAR T cells from human peripheral blood mononuclear cells. We then detail a three-step staining protocol with metal-labeled antibodies and the subsequent mass cytometry analysis. This protocol allows simultaneous characterization of CAR T cell intracellular signaling, activation, proliferation, cytokine production, and phenotype in a single assay.
| Type: | Article |
|---|---|
| Title: | High-dimensional functional phenotyping of preclinical human CAR T cells using mass cytometry |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.xpro.2022.101174 |
| Publisher version: | https://doi.org/10.1016/j.xpro.2022.101174 |
| Language: | English |
| Additional information: | © 2022 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10143508 |
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