Thippu Jayaprakash, Kamalram;
Mostafa, Denis;
Hussein, Mohammad;
Shaffer, Richard;
Michael, Agnieszka;
Ajaz, Mazhar;
Nisbet, Andrew;
(2022)
A High-Throughput In Vitro Radiobiology Platform for Megavoltage Photon Linear Accelerator Studies.
Applied Sciences
, 12
(3)
, Article 1456. 10.3390/app12031456.
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Abstract
We designed and developed a multiwell tissue culture plate irradiation setup, and intensity modulated radiotherapy plans were generated for 96-, 24-, and 6-well tissue culture plates. We demonstrated concordance between planned and measured/imaged radiation dose profiles using radiochromic film, a 2D ion chamber array, and an electronic portal-imaging device. Cell viability, clonogenic potential, and γ-H2AX foci analyses showed no significant differences between intensity-modulated radiotherapy and open-field, homogeneous irradiations. This novel platform may help to expedite radiobiology experiments within a clinical environment and may be used for wide-ranging ex vivo radiobiology applications.
| Type: | Article |
|---|---|
| Title: | A High-Throughput In Vitro Radiobiology Platform for Megavoltage Photon Linear Accelerator Studies |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3390/app12031456 |
| Publisher version: | https://doi.org/10.3390/app12031456 |
| Language: | English |
| Additional information: | This is an open access article distributed under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | intensity-modulated radiotherapy; in vitro radiobiology platform; personalised radiotherapy; preclinical radiotherapy studies; radiation-drug combinations |
| UCL classification: | UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng UCL > Provost and Vice Provost Offices > UCL BEAMS UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10143479 |
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