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The embryonic node behaves as an instructive stem cell niche for axial elongation

Solovieva, Tatiana; Lu, Hui-Chun; Moverley, Adam; Plachta, Nicolas; Stern, Claudio D; (2022) The embryonic node behaves as an instructive stem cell niche for axial elongation. Proceedings of the National Academy of Sciences , 119 (5) , Article e2108935119. 10.1073/pnas.2108935119. Green open access

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Abstract

In warm-blooded vertebrate embryos (mammals and birds), the axial tissues of the body form from a growth zone at the tail end, Hensen’s node, which generates neural, mesodermal, and endodermal structures along the midline. While most cells only pass through this region, the node has been suggested to contain a small population of resident stem cells. However, it is unknown whether the rest of the node constitutes an instructive niche that specifies this self-renewal behavior. Here, we use heterotopic transplantation of groups and single cells and show that cells not destined to enter the node can become resident and self-renew. Long-term resident cells are restricted to the posterior part of the node and single-cell RNA-sequencing reveals that the majority of these resident cells preferentially express G2/M phase cell-cycle–related genes. These results provide strong evidence that the node functions as a niche to maintain self-renewal of axial progenitors.

Type: Article
Title: The embryonic node behaves as an instructive stem cell niche for axial elongation
Open access status: An open access version is available from UCL Discovery
DOI: 10.1073/pnas.2108935119
Publisher version: https://doi.org/10.1073/pnas.2108935119
Language: English
Additional information: This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).
Keywords: Hensen’s node, stem cell niche, self-renewal, primitive streak, tail bud
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
URI: https://discovery.ucl.ac.uk/id/eprint/10143013
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