Lamb, J;
Šimaitis, J;
Halukeerthi, SO;
Salzmann, CG;
Holland, JP;
(2021)
Graphene Nanoflake Antibody Conjugates for Multimodal Imaging of Tumors.
Advanced NanoBiomed Research
, 1
(9)
, Article 2100009. 10.1002/anbr.202100009.
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Abstract
Graphene-based materials are promising scaffolds for use in the design of tailored-made nanomedicines. Herein, the synthesis and characterization of a series of multifunctional carboxylated graphene nanoflakes (GNFs) conjugated to monoclonal antibodies (mAbs) for tumor-specific binding and modulation of pharmacokinetics is presented. GNF–mAb constructs are coupled to a fluorophore (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene [BODIPY]) for applications in optical imaging, a paramagnetic Gd3+ complex, [GdDOTAGA(H2O)]−, and the hexadentate chelate desferrioxamine B (DFO) for radiolabeling with 89Zr4+ (t1/2 = 78.41 h) ions and applications in dual-modality positron emission tomography/magnetic resonance imaging (PET/MRI). Experimental properties of [89Zr]GdDOTAGA–ZrDFO–GNF–trastuzumab are tested in extensive chemical, spectroscopic, radiochemical, and cellular assays in vitro, and assessment of the pharmacokinetics by PET imaging in mice bearing a human ovarian cancer model illustrates the potential of using GNF–mAbs to develop multifunctional PET/MRI probes.
Type: | Article |
---|---|
Title: | Graphene Nanoflake Antibody Conjugates for Multimodal Imaging of Tumors |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.1002/anbr.202100009 |
Publisher version: | https://doi.org/10.1002/anbr.202100009 |
Language: | English |
Additional information: | Copyright © 2021 The Authors. Advanced NanoBiomed Research published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | antibodies, cancer, graphene, multimodal imaging, positron emission tomography |
UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
URI: | https://discovery.ucl.ac.uk/id/eprint/10142512 |
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