Kaynezhad, P;
Fosbury, R;
Hogg, C;
Tachtsidis, I;
Sivaprasad, S;
Jeffery, G;
(2022)
Near infrared spectroscopy reveals instability in retinal mitochondrial metabolism and haemodynamics with blue light exposure at environmental levels.
Journal of Biophotonics
, Article e2916. 10.1002/jbio.202100283.
(In press).
Preview |
Text
Tachtsidis_vor_Journal of Biophotonics - 2022 - Kaynezhad - Near infrared spectroscopy reveals instability in retinal mitochondrial.pdf - Published Version Download (703kB) | Preview |
Abstract
Blue light (~400-470nm) is considered potentially detrimental to the retina but is present in natural environmental light. Mitochondrial density is highest in the retina, and they exhibit a prominent optical absorption around 420nm arising from the Soret band of their porphyrins, including in cytochrome-c-oxidase in their respiratory chain. We examine the impact of continuous 420nm at environmental energy levels on retinal mitochondrial metabolism and haemodynamics in vivo in real time using broadband near-infrared spectroscopy. 1h environmental exposure to 420nm induces significant metabolic instability in retinal mitochondria and blood signals, which continues for up to 1h post blue exposure. Porphyrins are important in mitochondrial adenosine triphosphate (ATP) production and cytochrome-c-oxidase is a key part of the electron transport chain through which this is achieved. Hence, environmental 420nm likely restricts respiration and ATP production that may impact on retinal function. This article is protected by copyright. All rights reserved.
| Type: | Article |
|---|---|
| Title: | Near infrared spectroscopy reveals instability in retinal mitochondrial metabolism and haemodynamics with blue light exposure at environmental levels |
| Location: | Germany |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/jbio.202100283 |
| Publisher version: | https://doi.org/10.1002/jbio.202100283 |
| Language: | English |
| Additional information: | © 2022 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
| Keywords: | Blue light, Cytochrome-c-oxidase, Environmental light, Mitochondria, Near-Infrared Spectroscopy |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10142425 |
Archive Staff Only
 |
View Item |
