Cossarizza, A;
Chang, H-D;
Radbruch, A;
Abrignani, S;
Addo, R;
Akdis, M;
Andrae, I;
... Yang, J; + view all
(2021)
Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).
European Journal of Immunology
, 51
(12)
pp. 2708-3145.
10.1002/eji.202170126.
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Eur J Immunol - 2021 - Cossarizza - Guidelines for the use of flow cytometry and cell sorting in immunological studies .pdf - Published Version Download (98MB) | Preview |
Abstract
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.
| Type: | Article |
|---|---|
| Title: | Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/eji.202170126 |
| Publisher version: | https://doi.org/10.1002/eji.202170126 |
| Language: | English |
| Additional information: | © 2021 The Authors. European Journal of Immunology published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Immunology, REGULATORY T-CELLS, INNATE LYMPHOID-CELLS, HEMATOPOIETIC STEM-CELLS, MHC CLASS-I, NATURAL-KILLER-CELLS, ANTIGEN-SPECIFIC CD4(+), HUMAN BONE-MARROW, HUMAN B-CELL, ROR-GAMMA-T, HIGH-DIMENSIONAL CYTOMETRY |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10142329 |
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