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Monitoring tissue engineered constructs and protocols with laboratory based x-ray phase contrast tomography

Savvidis, S; Gerli, MFM; Pellegrini, M; Massimi, L; Hagen, CK; Endrizzi, M; Atzeni, A; ... Olivo, A; + view all (2022) Monitoring tissue engineered constructs and protocols with laboratory based x-ray phase contrast tomography. Acta Biomaterialia , 141 pp. 190-199. 10.1016/j.actbio.2022.01.022. Green open access

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Abstract

Tissue engineering (TE) aims to generate bioengineered constructs which can offer a surgical treatment for many conditions involving tissue or organ loss. Construct generation must be guided by suitable assessment tools. However, most current tools (e.g. histology) are destructive, which restricts evaluation to a single-2D anatomical plane, and has no potential for assessing constructs prior to or following their implantation. An alternative can be provided by laboratory-based x-ray phase contrast computed tomography (PC-CT), which enables the extraction of 3D density maps of an organ's anatomy. In this work, we developed a semi-automated image processing pipeline dedicated to the analysis of PC-CT slices of oesophageal constructs. Visual and quantitative (density and morphological) information is extracted on a volumetric basis, enabling a comprehensive evaluation of the regenerated constructs. We believe the presented tools can enable the successful regeneration of patient-specific oesophagus, and bring comparable benefit to a wide range of TE applications.

Type: Article
Title: Monitoring tissue engineered constructs and protocols with laboratory based x-ray phase contrast tomography
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.actbio.2022.01.022
Publisher version: https://doi.org/10.1016/j.actbio.2022.01.022
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: 3D-imaging; Laboratory-based phase contrast computed tomography (PC-CT); Regenerative medicine; In-vitro organ maturation
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Biology and Cancer Dept
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng
URI: https://discovery.ucl.ac.uk/id/eprint/10141920
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