Savvidis, S;
Gerli, MFM;
Pellegrini, M;
Massimi, L;
Hagen, CK;
Endrizzi, M;
Atzeni, A;
... Olivo, A; + view all
(2022)
Monitoring tissue engineered constructs and protocols with laboratory based x-ray phase contrast tomography.
Acta Biomaterialia
, 141
pp. 190-199.
10.1016/j.actbio.2022.01.022.
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Abstract
Tissue engineering (TE) aims to generate bioengineered constructs which can offer a surgical treatment for many conditions involving tissue or organ loss. Construct generation must be guided by suitable assessment tools. However, most current tools (e.g. histology) are destructive, which restricts evaluation to a single-2D anatomical plane, and has no potential for assessing constructs prior to or following their implantation. An alternative can be provided by laboratory-based x-ray phase contrast computed tomography (PC-CT), which enables the extraction of 3D density maps of an organ's anatomy. In this work, we developed a semi-automated image processing pipeline dedicated to the analysis of PC-CT slices of oesophageal constructs. Visual and quantitative (density and morphological) information is extracted on a volumetric basis, enabling a comprehensive evaluation of the regenerated constructs. We believe the presented tools can enable the successful regeneration of patient-specific oesophagus, and bring comparable benefit to a wide range of TE applications.
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