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Diffusional microfluidics for protein analysis

Xie, H; Yang, Y; Xia, C; Lee, TC; Pu, Q; Lan, Y; Zhang, Y; (2022) Diffusional microfluidics for protein analysis. TrAC - Trends in Analytical Chemistry , 146 , Article 116508. 10.1016/j.trac.2021.116508.

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Abstract

Wastewater-based epidemiology (WBE) is an emerging tool to monitor public health and the outbreak of infective diseases. It requires real-time information by measuring chemicals or biomarkers in wastewater generated by people at community-level for WBE analysis. Protein-based biomarkers provide valuable insights into community public health. However, due to the complexity of wastewater, the bottleneck of protein analysis in WBE lies in the lack of high-sensitive, cost-efficient and high-throughput analytical techniques. Diffusional microfluidics, which involves co-flowing streams of analytes and buffer under laminar flow conditions, allows the detection and analysis of biomolecules in their native state via the study of their diffusive behaviour in flow. In this review, recently developed analytical techniques, microfluidic diffusional sizing, free-flow electrophoresis, and hydrodynamic focusing microfluidic mixer, that are based on diffusional microfluidics are reviewed. Their applications in protein analysis including protein sizing, protein-protein interaction, protein separation and protein identification are highlighted.

Type: Article
Title: Diffusional microfluidics for protein analysis
DOI: 10.1016/j.trac.2021.116508
Publisher version: https://doi.org/10.1016/j.trac.2021.116508
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions.
Keywords: Diffusional microfluidics; Microfluidics; Protein analysis; Protein biomarker detection; Wastewater-based epidemiology
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > MAPS Faculty Office
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > MAPS Faculty Office > Institute for Materials Discovery
URI: https://discovery.ucl.ac.uk/id/eprint/10140979
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