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Tracking neuronal motility in live murine retinal explants

Aghaizu, ND; Warre-Cornish, KM; Robinson, MR; Ali, RR; Pearson, RA; (2021) Tracking neuronal motility in live murine retinal explants. STAR Protocols , 2 (4) , Article 101008. 10.1016/j.xpro.2021.101008. Green open access

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Abstract

The developing retina undergoes dynamic organizational changes involving significant intra-retinal motility of the encompassing cells. Here, we present a protocol for tracking retinal cell motility in live explanted mouse retinae. Although originally applied to rod and cone photoreceptors, this strategy is applicable to any fluorescently labeled cell in mouse retinae and other similar experimental retinal models. Careful tissue handling is critical for the successful acquisition of high-quality live imaging data. Further instructions for semi-automated in silico data handling are provided. For complete details on the use and execution of this protocol, please refer to Aghaizu et al. (2021).

Type: Article
Title: Tracking neuronal motility in live murine retinal explants
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.xpro.2021.101008
Publisher version: https://doi.org/10.1016/j.xpro.2021.101008
Language: English
Additional information: Copyright © 2021 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Department of Neuromuscular Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/10140665
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