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Continuous ultrafiltration/diafiltration using a 3D‐printed two membrane single pass module

Tan, R; Franzreb, M; (2020) Continuous ultrafiltration/diafiltration using a 3D‐printed two membrane single pass module. Biotechnology and Bioengineering , 117 (3) pp. 654-661. 10.1002/bit.27233. Green open access

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Abstract

A 3D printed ultrafiltration/diafiltration (UF/DF) module is presented allowing the continuous, simultaneous concentration of retained (bio-)molecules and reduction or exchange of the salt buffer. Differing from the single-pass UF concepts known from the literature, DF operation does not require the application of several steps or units with intermediating dilution. In contrast, the developed module uses two membranes confining the section in which the molecules are concentrated while the sample is passing. Simultaneously to this concentration process, the two membranes allow a perpendicular in and outflow of DF buffer reducing the salt content in this section. The module showed the continuous concentration of a dissolved protein up to a factor of 4.6 while reducing the salt concentration down to 47% of the initial concentration along a flow path length of only 5 cm. Due to single-pass operation the module shows concentration polarization effects reducing the effective permeability of the applied membrane in case of higher concentration factors. However, because of its simple design and the capability to simultaneously run UF and DF processes in a single module, the development could be economically beneficial for small scale UF/DF applications.

Type: Article
Title: Continuous ultrafiltration/diafiltration using a 3D‐printed two membrane single pass module
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/bit.27233
Publisher version: https://doi.org/10.1002/bit.27233
Language: English
Additional information: © 2019 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Keywords: 3D-printing, continuous, SPDF, SPTFF, UFDF
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10140064
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