UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

The effect of exercise on the protein profile of rat knee joint intra- and extra-articular ligaments

Kharaz, YA; Birch, H; Chester, A; Alchorne, E; Simpson, D; Clegg, P; Comerford, E; (2021) The effect of exercise on the protein profile of rat knee joint intra- and extra-articular ligaments. Scandinavian Journal of Medicine & Science in Sports , 31 (11) pp. 2033-2043. 10.1111/sms.14023. Green open access

[thumbnail of Birch_Final version.pdf]
Preview
Text
Birch_Final version.pdf - Accepted Version

Download (283kB) | Preview

Abstract

Injuries to the intra-articular anterior cruciate ligament (ACL) and the extra-articular medial collateral ligament (MCL) result in significant knee joint instability, pain, and immobility. Moderate endurance-type exercise can increase ligament strength but little is known on the effect of short-term regular bouts of high-intensity exercise on the extracellular matrix (ECM) structure of knee ligaments. Therefore, this study aimed to identify the effect of short-term regular bouts high exercise on the proteome of the rat ACL and MCL using mass spectrometry. Sprague-Dawley male rats (n = 6) were split into control and exercise groups, and subjected to high-intensity training for four 4 weeks followed by proteomic analyses of the ACL and MCL. Knee joint health status was assessed using OARSI and a validated histological scoring system. Histopathological analyses demonstrated no significant changes in either in cruciate, collateral ligaments, or cartilage between the control and exercised knee joints. However, significant proteins were found to be more abundant in the exercised ACL compared to ACL control group but not between the exercised MCL and control MCL groups. The significant abundant proteins in ACL exercise groups were mostly cytoskeletal, ribosomal and enzymes with several abundant matrisomal proteins such as collagen proteins and proteoglycans being found in this group. In conclusion, our results indicate that short-term regular bouts of high-intensity exercise have an impact on the intra-articular ACL but not extra-articular MCL ECM protein expression.

Type: Article
Title: The effect of exercise on the protein profile of rat knee joint intra- and extra-articular ligaments
Open access status: An open access version is available from UCL Discovery
DOI: 10.1111/sms.14023
Publisher version: https://doi.org/10.1111/sms.14023
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: ACL, exercise, MCL, proteomics, ANTERIOR CRUCIATE LIGAMENT, MEDIAL COLLATERAL LIGAMENT, HISTOLOGICAL ASSESSMENTS, BINDING-PROTEINS, TENDON, INJURY, EXPRESSION, RECOMMENDATIONS, OSTEOARTHRITIS, ADAPTATION
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science
URI: https://discovery.ucl.ac.uk/id/eprint/10139248
Downloads since deposit
91Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item