UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy

Johnson, T; Jones, K; Iacoviello, F; Turner, S; Jackson, N; Zourna, K; Welsh, J; ... Bracewell, D; + view all (2021) Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy. Membranes , 11 (11) , Article 905. 10.3390/membranes11110905. Green open access

[thumbnail of membranes-11-00905-v2.pdf]
Preview
Text
membranes-11-00905-v2.pdf - Published Version

Download (5MB) | Preview

Abstract

Two high resolution, 3D imaging techniques were applied to visualize and characterize sterilizing grade dual-layer filtration of liposomes, enabling membrane structure to be related with function and performance. Two polyethersulfone membranes with nominal retention ratings of 650 nm and 200 nm were used to filter liposomes of an average diameter of 143 nm and a polydispersity index of 0.1. Operating conditions including differential pressure were evaluated. X-ray computed tomography at a pixel size of 63 nm was capable of resolving the internal geometry of each membrane. The respective asymmetry and symmetry of the upstream and downstream membranes could be measured, with pore network modeling used to identify pore sizes as a function of distance through the imaged volume. Reconstructed 3D digital datasets were the basis of tortuous flow simulation through each porous structure. Confocal microscopy visualized liposome retention within each membrane using fluorescent dyes, with bacterial challenges also performed. It was found that increasing pressure drop from 0.07 MPa to 0.21 MPa resulted in differing fluorescent retention profiles in the upstream membrane. These results highlighted the capability for complementary imaging approaches to deepen understanding of liposome sterilizing grade filtration.

Type: Article
Title: Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy
Open access status: An open access version is available from UCL Discovery
DOI: 10.3390/membranes11110905
Publisher version: https://doi.org/10.3390/membranes11110905
Language: English
Additional information: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Keywords: Sterile Filtration, Liposomes, X-ray computed tomography, Confocal microscopy
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10139224
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item