Johnson, T;
Jones, K;
Iacoviello, F;
Turner, S;
Jackson, N;
Zourna, K;
Welsh, J;
... Bracewell, D; + view all
(2021)
Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy.
Membranes
, 11
(11)
, Article 905. 10.3390/membranes11110905.
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Abstract
Two high resolution, 3D imaging techniques were applied to visualize and characterize sterilizing grade dual-layer filtration of liposomes, enabling membrane structure to be related with function and performance. Two polyethersulfone membranes with nominal retention ratings of 650 nm and 200 nm were used to filter liposomes of an average diameter of 143 nm and a polydispersity index of 0.1. Operating conditions including differential pressure were evaluated. X-ray computed tomography at a pixel size of 63 nm was capable of resolving the internal geometry of each membrane. The respective asymmetry and symmetry of the upstream and downstream membranes could be measured, with pore network modeling used to identify pore sizes as a function of distance through the imaged volume. Reconstructed 3D digital datasets were the basis of tortuous flow simulation through each porous structure. Confocal microscopy visualized liposome retention within each membrane using fluorescent dyes, with bacterial challenges also performed. It was found that increasing pressure drop from 0.07 MPa to 0.21 MPa resulted in differing fluorescent retention profiles in the upstream membrane. These results highlighted the capability for complementary imaging approaches to deepen understanding of liposome sterilizing grade filtration.
| Type: | Article |
|---|---|
| Title: | Liposome Sterile Filtration Characterization via X-ray Computed Tomography and Confocal Microscopy |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3390/membranes11110905 |
| Publisher version: | https://doi.org/10.3390/membranes11110905 |
| Language: | English |
| Additional information: | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
| Keywords: | Sterile Filtration, Liposomes, X-ray computed tomography, Confocal microscopy |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10139224 |
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