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Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake

Smith, PO; Black, DJ; Pal, R; Avó, J; Dias, FB; Linthwaite, VL; Cann, MJ; (2021) Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake. Frontiers in Chemistry , 9 , Article 743928. 10.3389/fchem.2021.743928. Green open access

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Abstract

A new method for facilitating the delivery, uptake and intracellular localisation of thermally activated delayed fluorescence (TADF) complexes was developed. First, confinement of TADF complexes in liposomes was demonstrated, which were subsequently used as the delivery vehicle for cellular uptake. Confocal fluorescence microscopy showed TADF complexes subsequently localise in the cytoplasm of HepG2 cells. The procedures developed in this work included the removal of molecular oxygen in the liposome preparation without disrupting the liposome structures. Time-resolved fluorescence microscopy (point scanning) showed initial prompt fluorescence followed by a weak, but detectable, delayed fluorescence component for liposomal TADF internalised in HepG2 cells. By demonstrating that it is possible to deliver un-functionalised and/or unshielded TADF complexes, a sensing function for TADFs, such as molecular oxygen, can be envisaged.

Type: Article
Title: Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake
Open access status: An open access version is available from UCL Discovery
DOI: 10.3389/fchem.2021.743928
Publisher version: https://doi.org/10.3389/fchem.2021.743928
Language: English
Additional information: © 2021 Smith, Black, Pal, Avó, Dias, Linthwaite, Cann and Pålsson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
Keywords: fluorescence microscopy, bioimaging, liposomes, thermally activated delayed fluorescence (TADF), sensing
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
URI: https://discovery.ucl.ac.uk/id/eprint/10138799
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