Jeremy, G;
Stevens, J;
Lowe, AR;
(2016)
Single-Molecule Imaging to Characterize the Transport Mechanism of the Nuclear Pore Complex.
In:
Chromosome Architecture.
(pp. 17-35).
Springer: New York, NY, USA.
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Abstract
In the eukaryotic cell, a large macromolecular channel, known as the Nuclear Pore Complex (NPC), mediates all molecular transport between the nucleus and cytoplasm. In recent years, single-molecule fluorescence (SMF) imaging has emerged as a powerful tool to study the molecular mechanism of transport through the NPC. More recently, techniques such as single-molecule localization microscopy (SMLM) have enabled the spatial and temporal distribution of cargos, transport receptors and even structural components of the NPC to be determined with nanometre accuracy. In this protocol, we describe a method to study the position and/or motion of individual molecules transiting through the NPC with high spatial and temporal precision.
| Type: | Book chapter |
|---|---|
| Title: | Single-Molecule Imaging to Characterize the Transport Mechanism of the Nuclear Pore Complex |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-4939-3631-1_3 |
| Publisher version: | https://doi.org/10.1007/978-1-4939-3631-1_3 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions. |
| Keywords: | Nuclear pore complex, Nucleus, Single-molecule tracking, Super-resolution microscopy, Cell Nucleus, Cytoplasm, HeLa Cells, Humans, Nuclear Pore Complex Proteins, Protein Transport, Single Molecule Imaging |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10138752 |
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