UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

A study on myogenesis by regulation of reactive oxygen species and cytotoxic activity by selenium nanoparticles

Lee, SC; Lee, NH; Patel, KD; Jun, SK; Park, JH; Knowles, JC; Kim, HW; ... Lee, JH; + view all (2021) A study on myogenesis by regulation of reactive oxygen species and cytotoxic activity by selenium nanoparticles. Antioxidants , 10 (11) , Article 1727. 10.3390/antiox10111727. Green open access

[thumbnail of antioxidants-10-01727-v2.pdf]
Preview
Text
antioxidants-10-01727-v2.pdf - Published Version

Download (5MB) | Preview

Abstract

Reactive oxygen species (ROS) are continuously produced by skeletal muscle during con-tractile activity and even at rest. However, the ROS generated from excessive exercise or traumatic damage may produce more ROS than can be neutralized by an antioxidant capacity, which can be harmful to muscle function. In particular, selenium is a known antioxidant that regulates physiological functions such as cell differentiation and anti-inflammatory function. In this study, we developed nano-sized antioxidative biomaterials using selenium to investigate the protective and differentiation effects against C2C12 myoblasts in an H2 O2-induced oxidative stress environment. The selenium nanoparticles (SeNPs) were produced with a size of 35.6 ± 4.3 nm and showed antioxidant effects according to the 3,3′,5,5′-tetramethylbenzidine assay. Then, SeNPs were treated to C2C12 cells with or without H2 O2 . Our results showed that SeNPs reduced C2C12 apoptosis and intracellular ROS levels. Additionally, SeNPs effectively up-regulated in the presence of H2 O2, MyoD, MyoG, α-actinin, and myosin heavy chain, which are well known to increase during myoblast differentiation as assayed by qRT-PCR, immunocytochemistry-staining, western blotting. These results demonstrate that SeNPs can accelerate differentiation with its protective effects from the ROS environment and can be applied to the treatment of skeletal muscle in a cellular redox environment.

Type: Article
Title: A study on myogenesis by regulation of reactive oxygen species and cytotoxic activity by selenium nanoparticles
Open access status: An open access version is available from UCL Discovery
DOI: 10.3390/antiox10111727
Publisher version: https://doi.org/10.3390/antiox10111727
Language: English
Additional information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Keywords: C2C12; skeletal muscle differentiation; selenium nanoparticle; ROS; antioxidant
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng
URI: https://discovery.ucl.ac.uk/id/eprint/10137892
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item