Liu, CJ;
Ammon, W;
Siless, V;
Fogarty, M;
Wang, R;
Atzeni, A;
Aganj, I;
... Wang, H; + view all
(2021)
Quantification of volumetric morphometry and optical property in the cortex of human cerebellum at micrometer resolution.
Neuroimage
, 244
, Article 118627. 10.1016/j.neuroimage.2021.118627.
Preview |
Text
1-s2.0-S1053811921009009-main.pdf - Published Version Download (4MB) | Preview |
Abstract
The surface of the human cerebellar cortex is much more tightly folded than the cerebral cortex. Volumetric analysis of cerebellar morphometry in magnetic resonance imaging studies suffers from insufficient resolution, and therefore has had limited impact on disease assessment. Automatic serial polarization-sensitive optical coherence tomography (as-PSOCT) is an emerging technique that offers the advantages of microscopic resolution and volumetric reconstruction of large-scale samples. In this study, we reconstructed multiple cubic centimeters of ex vivo human cerebellum tissue using as-PSOCT. The morphometric and optical properties of the cerebellar cortex across five subjects were quantified. While the molecular and granular layers exhibited similar mean thickness in the five subjects, the thickness varied greatly in the granular layer within subjects. Layer-specific optical property remained homogenous within individual subjects but showed higher cross-subject variability than layer thickness. High-resolution volumetric morphometry and optical property maps of human cerebellar cortex revealed by as-PSOCT have great potential to advance our understanding of cerebellar function and diseases.
| Type: | Article |
|---|---|
| Title: | Quantification of volumetric morphometry and optical property in the cortex of human cerebellum at micrometer resolution |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.neuroimage.2021.118627 |
| Publisher version: | https://doi.org/10.1016/j.neuroimage.2021.118627 |
| Language: | English |
| Additional information: | © 2021 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
| Keywords: | Cell density, Cerebellum, Cross-subject variability, Layer thickness, Myelin, Polarization-sensitive optical coherence tomography |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10137666 |
Archive Staff Only
 |
View Item |
