Mo, J;
Leung, N;
Gupta, P;
Zhu, B;
Velliou, E;
Sui, T;
(2021)
Novel in situ multi-level analysis of structural-mechanical relations in a bioinspired polyurethane-based tissue model.
Materials Today Advances
, 12
, Article 100184. 10.1016/j.mtadv.2021.100184.
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Abstract
In this manuscript, we elucidated, for the first time, the substructural mechanisms present in our recently developed bioinspired polyurethane-based pancreatic tissue models. Different protein coatings of the model, i.e., collagen and fibronectin were examined. More specifically, analysis took place by combined real-time synchrotron X-ray scattering techniques and confocal laser scanning microscopy, to quantify the structural alteration of uncoated-polyurethane (PU) and protein-coated PU as well as the time-resolved structural reorganisation occurring at the micro-, nano- and lattice length scales during in situ micromechanical testing. We demonstrate that a clear increase of stiffness at the lamellar level following the fibronectin-PU modification, which is linked to the changes in the mechanics of the lamellae and interlamellar cohesion. This multi-level analysis of structural-mechanical relations in this polyurethane-based pancreatic cancer tissue model opens an opportunity in designing mechanically robust cost-effective tissue models not only for fundamental research but also for treatment screening.
| Type: | Article |
|---|---|
| Title: | Novel in situ multi-level analysis of structural-mechanical relations in a bioinspired polyurethane-based tissue model |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.mtadv.2021.100184 |
| Publisher version: | https://doi.org/10.1016/j.mtadv.2021.100184 |
| Language: | English |
| Additional information: | © 2021 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) |
| Keywords: | Bioinspired polyurethane, Surface functionalisation, Synchrotron X-ray scattering techniques, Confocal laser scanning microscopy, In situ micromechanical testing, Pancreatic cancer, Cancer cells, 3D scaffolds, Extracellular matrix (ECM), Collagen, Fibronectin |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Targeted Intervention |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10137131 |
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