UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

A Bilayer Osteochondral Scaffold with Self‐Assembled Monomeric Collagen Type‐I, Type‐II, and Polymerized Chondroitin Sulfate Promotes Chondrogenic and Osteogenic Differentiation of Mesenchymal Stem Cells

Rashidi, N; Tamaddon, M; Liu, C; Brand, DD; Czernuszka, J; (2021) A Bilayer Osteochondral Scaffold with Self‐Assembled Monomeric Collagen Type‐I, Type‐II, and Polymerized Chondroitin Sulfate Promotes Chondrogenic and Osteogenic Differentiation of Mesenchymal Stem Cells. Advanced NanoBiomed Research , Article 2100089. 10.1002/anbr.202100089. (In press). Green open access

[thumbnail of Liu_anbr.202100089.pdf]
Preview
Text
Liu_anbr.202100089.pdf - Published Version

Download (1MB) | Preview

Abstract

Osteochondral (OC) injuries are suffered by over 40 million patients in Europe alone. Tissue-engineering approaches may provide a more promising alternative over current treatments by potentially eliminating the need for revision surgery and creating a long-term substitute. Herein, the goal is to capture the natural biological and mechanical properties of the joint by developing a bilayer scaffold that is novel in two ways: first, a biomimetic bottom-up approach is used to improve production precision and reduce immunogenicity; monomeric collagen type I and II are self-assembled to fibrils and then processed to 3D scaffolds. Second, to induce a tissue-specific response in mesenchymal stem cells (MSCs), polymerized chondroitin sulfate (PCS) is synthesized and grafted to collagen II and hydroxyapatite (HA) is added to collagen I. Incorporation of PCS into collagen II induces a chondrogenic response by upregulation of COL2A1 and ACAN expression, and incorporation of HA into collagen I stimulates osteogenesis and upregulates the expression of COL1A2 and RUNX2. It is remarkable that MSCs give rise to distinct behavior of chondrogenesis and osteogenesis in the two different regions of the bilayer scaffold. This hybrid scaffold of collagen II-PCS and collagen I-HA offers a great potential treatment for OC injuries.

Type: Article
Title: A Bilayer Osteochondral Scaffold with Self‐Assembled Monomeric Collagen Type‐I, Type‐II, and Polymerized Chondroitin Sulfate Promotes Chondrogenic and Osteogenic Differentiation of Mesenchymal Stem Cells
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/anbr.202100089
Publisher version: https://doi.org/10.1002/anbr.202100089
Language: English
Additional information: © 2021 The Authors. Advanced NanoBiomed Research published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords: Bilayer osteochondral scaffolds, bone tissue engineering, cartilage tissue engineering, chondroitin sulphate polymerization, collagen scaffolds, mesenchymal stem cells, osteochondral tissue engineering
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science
URI: https://discovery.ucl.ac.uk/id/eprint/10136935
Downloads since deposit
73Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item