Hollósi, A;
Pászty, K;
Kellermayer, M;
Charras, G;
Varga, A;
(2021)
BRAF Modulates Stretch-Induced Intercellular Gap Formation through Localized Actin Reorganization.
International Journal of Molecular Sciences
, 22
(16)
, Article 8989. 10.3390/ijms22168989.
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Abstract
Mechanical forces acting on cell–cell adhesion modulate the barrier function of endothelial cells. The actively remodeled actin cytoskeleton impinges on cell–cell adhesion to counteract external forces. We applied stress on endothelial monolayers by mechanical stretch to uncover the role of BRAF in the stress-induced response. Control cells responded to external forces by organizing and stabilizing actin cables in the stretched cell junctions. This was accompanied by an increase in intercellular gap formation, which was prevented in BRAF knockdown monolayers. In the absence of BRAF, there was excess stress fiber formation due to the enhanced reorganization of actin fibers. Our findings suggest that stretch-induced intercellular gap formation, leading to a decrease in barrier function of blood vessels, can be reverted by BRAF RNAi. This is important when the endothelium experiences changes in external stresses caused by high blood pressure, leading to edema, or by immune or cancer cells in inflammation or metastasis.
| Type: | Article |
|---|---|
| Title: | BRAF Modulates Stretch-Induced Intercellular Gap Formation through Localized Actin Reorganization |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3390/ijms22168989 |
| Publisher version: | https://doi.org/10.3390/ijms22168989 |
| Language: | English |
| Additional information: | This is an open access article distributed under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
| Keywords: | actin cytoskeleton; BRAF RNAi; endothelial monolayer; intercellular gaps; mechanical stretch |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10133768 |
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