UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Cx43 mediates changes in myofibroblast contraction and collagen release in human amniotic membrane defects after trauma

Costa, E; Okesola, BO; Thrasivoulou, C; Becker, DL; Deprest, JA; David, AL; Chowdhury, TT; (2021) Cx43 mediates changes in myofibroblast contraction and collagen release in human amniotic membrane defects after trauma. Scientific Reports , 11 (1) , Article 16975. 10.1038/s41598-021-94767-4. Green open access

[thumbnail of Published version]
Preview
Text (Published version)
Costa Sci Rep healing AM.pdf - Published Version

Download (9MB) | Preview
[thumbnail of Supplementary Material]
Preview
Text (Supplementary Material)
SupplementaryInformation.pdf - Published Version

Download (802kB) | Preview

Abstract

The wound healing capacity of the fetal membranes after spontaneous or iatrogenic membrane rupture is unclear. We examined the healing mechanisms in amniotic membrane (AM) defects after trauma. Traumatised human AM defects were cultured for 4 days. Markers for nuclear (DAPI), cell type (vimentin, αSMA) and healing (Cx43, TGFβ1, collagen) were examined by immunofluorescence (IMF) confocal microscopy, Second Harmonic Generation (SHG) imaging and RT-qPCR. After trauma, AMCs and myofibroblasts migrated to the AM wound edge. Within four days, αSMA expressing myofibroblasts showed abundant Cx43 localized in the cytoplasmic processes. The highly contractile spindle-shaped myofibroblasts were present in the defect site and released collagen. In contrast, AMCs expressed vimentin and formed Cx43 plaques between cells found in the outer edges of the wound. Whilst AMCs were absent in the defect site, αSMA expressing myofibroblasts continued to elongate and polarize the collagen fibres. Both TGFβ1 and Cx43 gene expression were significantly increased after trauma. Cx43 has differential effects on AM cell populations that increase cellularity, contraction and potentially migration to the wound edge resulting in collagen polarisation in the AM defect site. Establishing how Cx43 regulates AM cell function could be an approach to repair defects in the membranes after trauma.

Type: Article
Title: Cx43 mediates changes in myofibroblast contraction and collagen release in human amniotic membrane defects after trauma
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/s41598-021-94767-4
Publisher version: https://doi.org/10.1038/s41598-021-94767-4
Language: English
Additional information: © 2021 Springer Nature Limited. This article is licensed under a Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).
Keywords: Biomedical engineering, Trauma
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health > Maternal and Fetal Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/10133633
Downloads since deposit
42Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item