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Closely related Lak megaphages replicate in the microbiomes of diverse animals

Crisci, MA; Chen, L-X; Devoto, AE; Borges, AL; Bordin, N; Sachdeva, R; Tett, A; ... Santini, JM; + view all (2021) Closely related Lak megaphages replicate in the microbiomes of diverse animals. iScience , Article 102875. 10.1016/j.isci.2021.102875. (In press). Green open access

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Abstract

Lak phages with alternatively coded ∼540 kbp genomes were recently reported to replicate in Prevotella in microbiomes of humans that consume a non-western diet, baboons and pigs. Here, we explore Lak phage diversity and broader distribution using diagnostic PCR and genome-resolved metagenomics. Lak phages were detected in 13 animal types, including reptiles, and are particularly prevalent in pigs. Tracking Lak through the pig gastrointestinal tract revealed significant enrichment in the hindgut compared to the foregut. We reconstructed 34 new Lak genomes, including six curated complete genomes, all of which are alternatively coded. An anomalously large (∼660 kbp) complete genome reconstructed for the most deeply branched Lak from a horse microbiome is also alternatively coded. From the Lak genomes, we identified proteins associated with specific animal species; notably, most have no functional predictions. The presence of closely related Lak phages in diverse animals indicates facile distribution coupled to host-specific adaptation.

Type: Article
Title: Closely related Lak megaphages replicate in the microbiomes of diverse animals
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.isci.2021.102875
Publisher version: https://doi.org/10.1016/j.isci.2021.102875
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10131431
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