Schwenke, J;
Yusuf, M;
Shemilt, LA;
Wagner, U;
Sajid, A;
Morrison, GR;
Zhang, F;
... Robinson, IK; + view all
(2021)
Quantitative phase measurements of human cell nuclei using X-ray ptychography.
Journal of Synchrotron Radiation
, 28
(4)
pp. 1166-1173.
10.1107/S1600577521004586.
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Abstract
The human cell nucleus serves as an important organelle holding the genetic blueprint for life. In this work, X-ray ptychography was applied to assess the masses of human cell nuclei using its unique phase shift information. Measurements were carried out at the I13-1 beamline at the Diamond Light Source that has extremely large transverse coherence properties. The ptychographic diffractive imaging approach allowed imaging of large structures that gave quantitative measurements of the phase shift in 2D projections. In this paper a modified ptychography algorithm that improves the quality of the reconstruction for weak scattering samples is presented. The application of this approach to calculate the mass of several human nuclei is also demonstrated.
| Type: | Article |
|---|---|
| Title: | Quantitative phase measurements of human cell nuclei using X-ray ptychography |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1107/S1600577521004586 |
| Publisher version: | https://doi.org/10.1107/S1600577521004586 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions. |
| Keywords: | X-ray, nuclei, ptychography, quantitative phase imaging, synchrotron |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10131026 |
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