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Strategy for managing industrial anaerobic sludge through the heterotrophic cultivation of chlorella sorokiniana: Effect of iron addition on biomass and lipid production

Charria-Girón, E; Amazo, V; De Angulo, D; Hidalgo, E; Villegas-Torres, MF; Baganz, F; Ortega, NHC; (2021) Strategy for managing industrial anaerobic sludge through the heterotrophic cultivation of chlorella sorokiniana: Effect of iron addition on biomass and lipid production. Bioengineering , 8 (6) , Article 82. 10.3390/bioengineering8060082. Green open access

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Abstract

Microalgae provides an alternative for the valorization of industrial by-products, in which the nutritional content varies substantially and directly affects microalgae system performance. Herein, the heterotrophic cultivation of Chlorella sorokiniana was systematically studied, allowing us to detect a nutritional deficiency other than the carbon source through assessing the oxygen transfer rate for glucose or acetate fermentation. Consequently, a mathematical model of the iron co-limiting effect on heterotrophic microalgae was developed by exploring its ability to regulate the specific growth rate and yield. For instance, higher values of the specific growth rate (0.17 h−1 ) compared with those reported for the heterotrophic culture of Chlorella were obtained due to iron supplementation. Therefore, anaerobic sludge from an industrial wastewater treatment plant (a baker’s yeast company) was pretreated to obtain an extract as a media supplement for C. sorokiniana. According to the proposed model, the sludge extract allowed us to supplement iron values close to the growth activation concentration (KFe ~12 mg L−1). Therefore, a fed-batch strategy was evaluated on nitrogen-deprived cultures supplemented with the sludge extract to promote biomass formation and fatty acid synthesis. Our findings reveal that nitrogen and iron in sludge extract can supplement heterotrophic cultures of Chlorella and provide an alternative for the valorization of industrial anaerobic sludge.

Type: Article
Title: Strategy for managing industrial anaerobic sludge through the heterotrophic cultivation of chlorella sorokiniana: Effect of iron addition on biomass and lipid production
Open access status: An open access version is available from UCL Discovery
DOI: 10.3390/bioengineering8060082
Publisher version: http://dx.doi.org/10.3390/bioengineering8060082
Language: English
Additional information: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Keywords: anaerobic sludge; chlorella sorokiniana; heterotrophic cultivation; Iron; modeling
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10130738
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