UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Introducing Bacteria and Synthetic Biomolecules along Engineered DNA Fibers

Burns, JR; (2021) Introducing Bacteria and Synthetic Biomolecules along Engineered DNA Fibers. Small , 17 (25) , Article 2100136. 10.1002/smll.202100136. Green open access

[thumbnail of smll.202100136.pdf]
Preview
Text
smll.202100136.pdf - Published Version

Download (2MB) | Preview

Abstract

Deoxyribonucleic acid (DNA) nanotechnology enables user-defined structures to be built with unrivalled control. The approach is currently restricted across the nanoscale, yet the ability to generate macroscopic DNA structures has enormous potential with applications spanning material, physical, and biological science. To address this need, I employed DNA nanotechnology and developed a new macromolecular nanoarchitectonic assembly method to produce DNA fibers with customizable properties. The process involves coalescing DNA nanotubes under high salt conditions to yield filament superstructures. Using this strategy, fibers over 100 microns long, with stiffnesses 10 times greater than cytoskeletal actin filaments can be fabricated. The DNA framework enables fibers to be functionalized with advanced synthetic molecules, including, aptamers, origami, nanoparticles, and vesicles. In addition, the fibers can act as bacterial extracellular scaffolds and adhere Escherichia coli cells in a controllable fashion. These results showcase the opportunities offered from DNA nanotechnology across the macroscopic scale. The new biophysical approach should find widespread use, from the generation of hybrid-fabric materials, smart analytical devices in biomedicine, and platforms to study cell-cell interactions.

Type: Article
Title: Introducing Bacteria and Synthetic Biomolecules along Engineered DNA Fibers
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/smll.202100136
Publisher version: https://doi.org/10.1002/smll.202100136
Language: English
Additional information: © 2021 The Authors. Small published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords: Condensation, DNA, nanotechnology fibers, macromolecular nanoarchitectonics, nanotubes
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/10129762
Downloads since deposit
22Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item