Sergi, B;
Bulut, I;
Xia, Y;
Waller, ZAE;
Yildizhan, Y;
Acilan, C;
Lord, RM;
(2021)
Understanding the Potential In Vitro Modes of Action of Bis(beta-diketonato) Oxovanadium(IV) Complexes.
ChemMedChem
10.1002/cmdc.202100152.
(In press).
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Abstract
To understand the potential in vitro modes of action of bis(β-diketonato) oxovanadium(IV) complexes, nine compounds of varying functionality have been screened using a range of biological techniques. The antiproliferative activity against a range of cancerous and normal cell lines has been determined, and show these complexes are particularly sensitive against the lung carcinoma cell line, A549. Annexin V (apoptosis) and Caspase-3/7 assays were studied to confirm these complexes induce programmed cell death. While gel electrophoresis was used to determine DNA cleavage activity and production of reactive oxygen species (ROS), the Comet assay was used to determine induced genomic DNA damage. Additionally, Förster resonance energy transfer (FRET)-based DNA melting and fluorescent intercalation displacement assays have been used to determine the interaction of the complexes with double strand (DS) DNA and to establish preferential DNA base-pair binding (AT versus GC).
| Type: | Article |
|---|---|
| Title: | Understanding the Potential In Vitro Modes of Action of Bis(beta-diketonato) Oxovanadium(IV) Complexes |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/cmdc.202100152 |
| Publisher version: | https://doi.org/10.1002/cmdc.202100152 |
| Language: | English |
| Additional information: | © 2021 The Authors. ChemMedChem published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Apoptosis, β, -diketonate complexes, Bioinorganic Chemistry, DNA interactions, Vanadium complexes |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharma and Bio Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10129240 |
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