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FGF signalling plays similar roles in development and regeneration of the skeleton in the brittle star Amphiura filiformis

Czarkwiani, A; Dylus, DV; Carballo, L; Oliveri, P; (2021) FGF signalling plays similar roles in development and regeneration of the skeleton in the brittle star Amphiura filiformis. Development 10.1242/dev.180760. (In press). Green open access

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Abstract

Regeneration as an adult developmental process is in many aspects similar to embryonic development. Although many studies point out similarities and differences, no large-scale, direct and functional comparative analyses between development and regeneration of a specific cell type or structure in one animal exist. Here, we use the brittle star Amphiura filiformis to characterise the role of the FGF signalling pathway during skeletal development in embryos and arm regeneration. In both processes, we find ligands expressed in ectodermal cells flanking underlying skeletal mesenchymal cells, which express the receptors. Perturbation of FGF signalling showed inhibited skeleton formation in both embryogenesis and regeneration, without affecting other key developmental processes. Differential transcriptome analysis finds mostly differentiation genes rather than transcription factors to be downregulated in both contexts. Moreover, comparative gene analysis allowed us to discover brittle star specific, differentiation genes. In conclusion, our results show that the FGF pathway is crucial for skeletogenesis in the brittle star, as in other deuterostomes and provide evidence for the re-deployment of a developmental gene regulatory module during regeneration.

Type: Article
Title: FGF signalling plays similar roles in development and regeneration of the skeleton in the brittle star Amphiura filiformis
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/dev.180760
Publisher version: https://doi.org/10.1242/dev.180760
Language: English
Additional information: Copyright © 2021. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Keywords: Echinoderm, Biomineralization, Regulatory networks, Signalling, VegF
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: https://discovery.ucl.ac.uk/id/eprint/10128151
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