Hertweck, A;
Jenner, RG;
(2021)
The Study of Protein–DNA Interactions in CD4+ T-Cells Using ChIPmentation.
Methods in Molecular Biology
, 2285
pp. 201-216.
10.1007/978-1-0716-1311-5_17.
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Abstract
Chromatin immunoprecipitation (ChIP) coupled with high-throughput sequencing (ChIP-seq) is an invaluable method to profile of enrichment of histone modifications and transcription factor binding sites across the genome. However, standard ChIP-seq protocols require large numbers of cells (>10^7) as starting material, which are often impossible to obtain for rare immune populations. Here we describe a streamlined ChIP protocol optimised for small cell numbers in conjunction with transposon-tagging mediated sequencing library preparation (ChIPmentation) which allows the analysis of samples of as low as 10^5 cells.
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