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The Study of Protein–DNA Interactions in CD4+ T-Cells Using ChIPmentation

Hertweck, A; Jenner, RG; (2021) The Study of Protein–DNA Interactions in CD4+ T-Cells Using ChIPmentation. Methods in Molecular Biology , 2285 pp. 201-216. 10.1007/978-1-0716-1311-5_17. Green open access

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Abstract

Chromatin immunoprecipitation (ChIP) coupled with high-throughput sequencing (ChIP-seq) is an invaluable method to profile of enrichment of histone modifications and transcription factor binding sites across the genome. However, standard ChIP-seq protocols require large numbers of cells (>10^7) as starting material, which are often impossible to obtain for rare immune populations. Here we describe a streamlined ChIP protocol optimised for small cell numbers in conjunction with transposon-tagging mediated sequencing library preparation (ChIPmentation) which allows the analysis of samples of as low as 10^5 cells.

Type: Article
Title: The Study of Protein–DNA Interactions in CD4+ T-Cells Using ChIPmentation
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-0716-1311-5_17
Publisher version: https://doi.org/10.1007/978-1-0716-1311-5_17
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: ChIP, ChIPmentation, Chromatin immunoprecipitation, Transcription factor
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Cancer Bio
URI: https://discovery.ucl.ac.uk/id/eprint/10127826
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