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In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture.

Richardson, SE; Ghazanfari, R; Chhetri, J; Enver, T; Böiers, C; (2021) In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture. STAR Protocols , 2 (2) , Article 100420. 10.1016/j.xpro.2021.100420. Green open access

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Abstract

In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the in utero initiation of childhood B acute lymphoblastic leukemia. Herein, we detail critical aspects of the protocol including reagent validation, controls, and examples of surface markers used for analysis and cell sorting. For complete details on the use and execution of this protocol, please refer to Boiers et al. (2018).

Type: Article
Title: In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture.
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.xpro.2021.100420
Publisher version: https://doi.org/10.1016/j.xpro.2021.100420
Language: English
Additional information: © 2021 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: Cell Differentiation, Cell culture, Stem Cells
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Cancer Bio
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology
URI: https://discovery.ucl.ac.uk/id/eprint/10127219
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