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Nanoparticle tracking analysis as a process analytical tool for characterising magnetosome preparations

Fernández-Castané, A; Li, H; Joseph, S; Ebeler, M; Franzreb, M; Bracewell, DG; Overton, TW; (2021) Nanoparticle tracking analysis as a process analytical tool for characterising magnetosome preparations. Food and Bioproducts Processing , 127 pp. 426-434. 10.1016/j.fbp.2021.03.013. Green open access

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Abstract

Nanoparticle Tracking Analysis (NTA) has been employed to measure the particle concentration and size distribution of magnetosomes extracted and purified from Magnetospirillum gryphiswaldense MSR-1, and then exposed to probe ultrasonication for various times, or 1% (w/v) sodium dodecyl sulphate (SDS) for 1 h. Particle concentration increased 3.7-fold over the first 15 min of ultrasonication (from 2 × 108 to >7.3 × 108 particles mL−1), but fell steeply to ∼3.6 × 108 particles mL−1 after 20 min. NTA of untreated magnetosome preparation confirmed a wide particle distribution dominated by larger species (D[1,0] = 312 nm; Dn50 = 261 nm; mode = 243 nm) with no particles in the size range of isolated single magnetosomes. After 5 min of ultrasonication the whole particle size distribution shifted to smaller size (D[1,0] = 133 nm; Dn50 = 99 nm; mode = 36 nm, corresponding to individual magnetosomes), but longer treatment times (15 and 20 min) reversed the previous transition; all characteristic numbers of the particle size distributions increased and very few small particles were detected. Side-by-side comparison of NTA and TEM sizing data revealed remarkable similarity at low ultrasonication times, with both showing single magnetosomes accounted for ∼30% population after 5 min. Exposure of magnetosomes to SDS resulted in a ∼3-fold increase in particle concentration to 5.8 × 108 particles mL−1, narrowing of the size distribution and gross elimination of particles below 60 nm. We conclude that NTA is a rapid cost-effective technique for measuring particle concentration, size distribution and aggregation state of magnetosomes in solution.

Type: Article
Title: Nanoparticle tracking analysis as a process analytical tool for characterising magnetosome preparations
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.fbp.2021.03.013
Publisher version: https://doi.org/10.1016/j.fbp.2021.03.013
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Biomanufacturing, Bioprocessing, Magnetic nanoparticles, Nanobiotechnology, Process analytical technology, Quality control
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10125898
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