Fernández-Castané, A;
Li, H;
Joseph, S;
Ebeler, M;
Franzreb, M;
Bracewell, DG;
Overton, TW;
(2021)
Nanoparticle tracking analysis as a process analytical tool for characterising magnetosome preparations.
Food and Bioproducts Processing
, 127
pp. 426-434.
10.1016/j.fbp.2021.03.013.
Preview |
Text
1-s2.0-S0960308521000572-main.pdf - Accepted Version Download (3MB) | Preview |
Abstract
Nanoparticle Tracking Analysis (NTA) has been employed to measure the particle concentration and size distribution of magnetosomes extracted and purified from Magnetospirillum gryphiswaldense MSR-1, and then exposed to probe ultrasonication for various times, or 1% (w/v) sodium dodecyl sulphate (SDS) for 1 h. Particle concentration increased 3.7-fold over the first 15 min of ultrasonication (from 2 × 108 to >7.3 × 108 particles mL−1), but fell steeply to ∼3.6 × 108 particles mL−1 after 20 min. NTA of untreated magnetosome preparation confirmed a wide particle distribution dominated by larger species (D[1,0] = 312 nm; Dn50 = 261 nm; mode = 243 nm) with no particles in the size range of isolated single magnetosomes. After 5 min of ultrasonication the whole particle size distribution shifted to smaller size (D[1,0] = 133 nm; Dn50 = 99 nm; mode = 36 nm, corresponding to individual magnetosomes), but longer treatment times (15 and 20 min) reversed the previous transition; all characteristic numbers of the particle size distributions increased and very few small particles were detected. Side-by-side comparison of NTA and TEM sizing data revealed remarkable similarity at low ultrasonication times, with both showing single magnetosomes accounted for ∼30% population after 5 min. Exposure of magnetosomes to SDS resulted in a ∼3-fold increase in particle concentration to 5.8 × 108 particles mL−1, narrowing of the size distribution and gross elimination of particles below 60 nm. We conclude that NTA is a rapid cost-effective technique for measuring particle concentration, size distribution and aggregation state of magnetosomes in solution.
| Type: | Article |
|---|---|
| Title: | Nanoparticle tracking analysis as a process analytical tool for characterising magnetosome preparations |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.fbp.2021.03.013 |
| Publisher version: | https://doi.org/10.1016/j.fbp.2021.03.013 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Biomanufacturing, Bioprocessing, Magnetic nanoparticles, Nanobiotechnology, Process analytical technology, Quality control |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10125898 |
Archive Staff Only
 |
View Item |
