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Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure

Botchway, SW; Farooq, S; Sajid, A; Robinson, IK; Yusuf, M; (2021) Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure. Chromosome Research 10.1007/s10577-021-09654-5. (In press). Green open access

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Abstract

The organization of chromatin into higher-order structures and its condensation process represent one of the key challenges in structural biology. This is important for elucidating several disease states. To address this long-standing problem, development of advanced imaging methods has played an essential role in providing understanding into mitotic chromosome structure and compaction. Amongst these are two fast evolving fluorescence imaging technologies, specifically fluorescence lifetime imaging (FLIM) and super-resolution microscopy (SRM). FLIM in particular has been lacking in the application of chromosome research while SRM has been successfully applied although not widely. Both these techniques are capable of providing fluorescence imaging with nanometer information. SRM or “nanoscopy” is capable of generating images of DNA with less than 50 nm resolution while FLIM when coupled with energy transfer may provide less than 20 nm information. Here, we discuss the advantages and limitations of both methods followed by their contribution to mitotic chromosome studies. Furthermore, we highlight the future prospects of how advancements in new technologies can contribute in the field of chromosome science.

Type: Article
Title: Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/s10577-021-09654-5
Publisher version: https://doi.org/10.1007/s10577-021-09654-5
Language: English
Additional information: © 2021 Springer Nature Switzerland AG. This article is licensed under a Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).
Keywords: cytogenetics, chromosome chromatin, fluorescence microscopy, fluorescence lifetime imaging, time-resolved, super-resolution microscopy
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: https://discovery.ucl.ac.uk/id/eprint/10125249
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