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Gene Editing and Genotoxicity: Targeting the Off-Targets

Blattner, G; Cavazza, A; Thrasher, AJ; Turchiano, G; (2020) Gene Editing and Genotoxicity: Targeting the Off-Targets. Frontiers in Genome Editing , 2 , Article 613252. 10.3389/fgeed.2020.613252. Green open access

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Abstract

Gene editing technologies show great promise for application to human disease as a result of rapid developments in targeting tools notably based on ZFN, TALEN, and CRISPR-Cas systems. Precise modification of a DNA sequence is now possible in mature human somatic cells including stem and progenitor cells with increasing degrees of efficiency. At the same time new technologies are required to evaluate their safety and genotoxicity before widespread clinical application can be confidently implemented. A number of methodologies have now been developed in an attempt to predict expected and unexpected modifications occurring during gene editing. This review surveys the techniques currently available as state of the art, highlighting benefits and limitations, and discusses approaches that may achieve sufficient accuracy and predictability for application in clinical settings.

Type: Article
Title: Gene Editing and Genotoxicity: Targeting the Off-Targets
Open access status: An open access version is available from UCL Discovery
DOI: 10.3389/fgeed.2020.613252
Publisher version: https://doi.org/10.3389/fgeed.2020.613252
Language: English
Additional information: © 2020 Blattner, Cavazza, Thrasher and Turchiano. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/).
Keywords: gene editing, CRISPR, genotoxicity, off-target, DSB = double-strand break, DNA damage, translocation, chromosomal aberration
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept
URI: https://discovery.ucl.ac.uk/id/eprint/10125102
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