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Studies into the in vivo interactions between human immunodeficiency virus and human herpes virus 8

Carneiro Leao, Jair; (1999) Studies into the in vivo interactions between human immunodeficiency virus and human herpes virus 8. Doctoral thesis (Ph.D), UCL (University College London). Green open access

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Abstract

In HIV-coinfected people unaffected by Kaposi sarcoma (KS), circulating HHV-8 DMA and anti-HHV-8 antibodies are detected particularly frequently and at high levels. Studies were conducted to determine whether HHV-8 hyperactivity in such individuals could be the consequence of immunosuppression-associated reactivation, transactivation by HIV or the propensity of the host to multiple HHV-8 Infection. HHV-8 subgenomic DMA was amplified by PCR from peripheral blood of HIV-infected patients, health care workers and patients who had undergone bone marrow transplantation (BMT), and from oral lesional tissues of patients with AIDS-associated KS. Clones derived from amplicons originating from DMA fragments in open reading frame (ORF) 26 and ORF K1 were Isolated. For each ORF, intra-specimen nucleotide sequence differences were determined. The extent of HHV-8 variation in clones derived from blood of HIV-positive patients was significantly higher than In blood from health care workers or post-BMT patients or in AIDS-KS lesional tissue. Among the clones derived from the latter three categories of specimens, sequence variations were not significantly different. To Investigate if HIV plays a role in inducing HHV-8 hyperactivity, the frequency of circulating HHV-8 DMA, HIV load, anti-HHV-8 antibody level and CD4+ T cell counts was studied before and after therapy with protease inhibitors. While there was an increase in the CD4+ T cell counts and a decrease in HHV-8 DMA, no significant differences in the other variables were observed. The findings of this study suggest that HIV-infected individuals tend to be multiply-infected with HHV-8, that each AIDS-KS lesion is associated with infection by a single HHV-8 variant or a small group of related variants, and that HIV may Influence, or be associated with the replication of HHV-8.

Type: Thesis (Doctoral)
Qualification: Ph.D
Title: Studies into the in vivo interactions between human immunodeficiency virus and human herpes virus 8
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Thesis digitised by ProQuest.
URI: https://discovery.ucl.ac.uk/id/eprint/10124816
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